SERVALight Eos CL HRP WB Substrate Kit (42585.02)

Very highly sensitive enhanced chemiluminescence kit for the detection of immobilized proteins (Western Blot) or immobilized nucleic acids (Southern and Northern Blot) labelled directly with horseradish peroxidase (HRP) or indirectly with HRP-labelled antibodies/streptavidin. The substrate is readily prepared by mixing component A (luminol/enhancer solution) with component B (stabilized peroxide solution) in a one-to-one ratio.· Very high sensitivity, high femtogram limit of detection· Very long and steady light emission for 12 hours· Primary antibody dilution 1:1000 -1:15.000· Secondary antibody dilution 1:25.000 – 1:150.000· Detection can be done by film or CCD imaging equipmentSERVALight is produced by Cyanagen Srl. Cyanagen Srl is subject of US and EU patent application number US7803573; EP1962095; US7855287; EP1950207, together with other equivalent granted patents and patent applications in other countries.