Salt Active Nuclease solution in 50 % glycerol (18541.01)

Supplied as solution in 25 mM Tris-HCl pH 7.5, 5 mM MgCl2, 0.5 M NaCl, 0.01 % Triton® X-100, 50 % (v/v) glycerol.Salt Active Nuclease is a highly active non-specific endonuclease from a marine bacterium that cleaves both DNA and RNA. It digests DNA versus RNA in a 10:1 ratio.Salt Active Nuclease has optimum activity at 0.5 M NaCl, is active in the pH range of 7 to 10 and low temperatures, which makes the enzyme ideal for use in removal of nucleic acids from cell extracts and proteins samples. It will remove contaminating nucleic acids in a traditional protein buffer system. That guarantees the full protection of proteins while the nucleic acids are fully removed.• Non-specific endonuclease • Optimum activity at high salt concentration (0.5 M NaCl) • Active at low temperatures (20 % at 6 ºC) • Broad pH range • Easily inactivated by reducing agents