Rhodamine fibronectin (FNR01-GRP)

Product Uses Include

• Tracking cell movement through a 3D matrix
• Tracking cell movement across a glass slide
• Observation of fibronectin matrix assembly and cell adhesion.
• FACS analysis of fibronectin binding cells

Material
Fibronectin purified from bovine plasma and has been modified to contain a covalently linked rhodamine fluorescent dye. An activated ester of rhodamine has been used to label the protein with a labeling stoichiometry of apprximately 1.0 dyes per protein molecule, a low labeling stiochiometry to retain functional activity. No free dye is apparent in the final product. Fibronectin has an approximate molecular weight of 250 kDa. FNR01 (20 µg of protein) is provided as a lyophilized powder.

Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >90% pure. No free dye is apparent in the final product.