RhoA G-LISA GTPase Activation Assay Kit (Colorimetric format) (BK124-GRP)

Product Uses Include

• Rho signaling pathway studies
• Rho activation assays with primary cells
• Studies of Rho activators and inactivators
• Rho activation assays with limited material
• High throughput screens for Rho activation

Introduction
The G-LISA Rho activation assays are ELISA based Rho activation assays with which you can measure Rho activity in cells in less than 3 h. BK124 is very sensitive and has excellent accuracy between duplicate samples. The BK124 Rho activation assay kit measures the level of GTP-loaded RhoA only in cells. The level of activation is measured with absorbance set at 490nm. For a kit to measure RhoA activation with luminescence detection, see Cat. # BK121.

Kit contents

The kit contains sufficient reagents to perform 96 RhoA activation assays. Since the Rho-GTP affinity wells are supplied as strips and the strips can be broken into smaller pieces, each kit can be used for anywhere from one to multiple assays. The following components are included in the kit:

1. Rho-GTP affinity wells (12 strips of 8 wells)
2. Lysis buffer
3. Binding buffer
4. Antigen presenting buffer
5. Wash buffer
6. Antibody dilution buffer
7. Anti-RhoA antibody
8. HRP-labeled secondary antibody
9. Positive control RhoA protein
10. Protease inhibitor cocktail (Cat. # PIC02)
11. Absorbance detection reagents
12. Precision Red™ Advanced protein assay reagent (Cat. # ADV02)
13. Manual with detailed protocols and extensive troubleshooting guide