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Recombinant Human IFN-gamma Protein

Recombinant Human IFN-gamma Protein (RP01038-GRP)

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Description

Recombinant Human IFN-gamma Protein is produced by E. coli expression system. The target protein is expressed with sequence ( Gln24-Gln166) of human Interferon Gamma (Accession #NP_000610.2).

Specifications

Manufacturer Abclonal Biotechnology
Manufacturer Cat# RP01038
Bio-Activity 1.Measured by its binding ability in a functional ELISA. Immobilized Human FNGR1/CD119 at 1 μg/mL (100 μL/well) can bind Human IFNG with a linear range of 0.98-1.97 ng/mL.|2.Measured in a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 0.74-2.96 ng/mL, corresponding to a specific activity of 3.38×105~1.35×106 units/mg. |3.Flow cytometry: 1X106 A549 cells treated with IFN-γ (RP01038,1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (A26692,5 μl/Test,right).4.Flow cytometry: 1X106 NCI-H460 cells treated with IFN-γ (RP01038,1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (A26692,5 μl/Test,right)
Alternate Names IFNG,IFG,IFI
Reactivity Human
Gene ID 3458
Swiss Prot P01579-1