Recombinant Human IL-12/IL-12A&IL-12B Protein
Recombinant Human IL-12/IL-12A&IL-12B Protein (RP01232-GRP)
Products
Description
Active Recombinant Human IL-12A&IL-12B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile23-Ser328&Arg23-Ser219) of human IL-12 (Accession #NP_002178.2/NP_000873.2) fused with a 6×His tag at the C-terminus(IL12B),Flag tag at the C-terminus(IL12A).
Specifications
| Manufacturer | Abclonal Biotechnology |
|---|---|
| Manufacturer Cat# | RP01232 |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL-12 at 1 μg/mL (100 μL/well) can bind Recombinant Human IL12Rβ1 with a linear range of 30.6-122.4 ng/mL.|2.Measured in a cell proliferation assay using anti-CD3 antibody activated Human T lymphocyte leukemia cells (jurkat). The ED50 for this effect is typically 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×106-1.0×107units/mg.|3.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2-8 ng/mL, corresponding to a specific activity of 1.25×105~5×105 units/mg. |
| Alternate Names | CLMF,IL12,p70,Interleukin-12,IL12A,P35,NKSF |
| Reactivity | Human |
| Gene ID | 3592&3593 |
| Swiss Prot | P29459&P29460 |