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Recombinant Human IL-1 beta Protein

Recombinant Human IL-1 beta Protein (RP00002-GRP)

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Description

Recombinant Human IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala117-Ser269) of human IL-1 beta (Accession #NP_000567.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.

Specifications

Manufacturer Abclonal Biotechnology
Manufacturer Cat# RP00002
Bio-Activity 1.Recombinant Human IL1β protein was measured by NF-κB reporter assay in HEK293 cells. The ED50 for this effect is 1-10 pg/mL, corresponding to a specific activity of 1×108~1×109 units/mg.|2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.217-0.870 ng/mL, corresponding to a specific activity of 1.15×106~4.61×106 units/mg.|3.Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta (Catalog: RP00002) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 (Catalog: RP01036) with a linear range of 0.002-1 μg/mL.
Alternate Names IL-1,IL1-BETA,IL1F2,IL1 beta,IL1B
Reactivity Human
Gene ID 3553
Swiss Prot P01584