Recombinant Human IGF-I Protein
Recombinant Human IGF-I Protein (RP00996-GRP)
Products
Description
Recombinant Human IGF-I Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence ( Gly 49 - Ala 118) of human IGF1 (Accession #NP_001104755.1) fused with an Fc, 6×His tag at the C-terminus.
Specifications
| Manufacturer | Abclonal Biotechnology |
|---|---|
| Manufacturer Cat# | RP00996 |
| Bio-Activity | 1.Measured by its ability to stimulate p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells. 0.01-1 ng/mL of Recombinant Human IGF1 can effectively enhance p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.|2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1 μg/mL (100 μL/well) can bind recombinant human IGF1 with a linear range of 30-250 ng/mL. 3.Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 7.5-30 ng/mL, corresponding to a specific activity of 3.33×104-1.33×105units/mg. |
| Alternate Names | IGF1,IGF-I,IGFI,MGF, IGFI, MGF |
| Reactivity | Human |
| Gene ID | 3479 |
| Swiss Prot | P05019-1 |