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Recombinant Human FGF-2/bFGF Protein

Recombinant Human FGF-2/bFGF Protein (RP01042-GRP)

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Description

Recombinant Human FGF-2/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro143-Ser288) of human FGF2 (Accession #NP_001997.5).

Specifications

Manufacturer Abclonal Biotechnology
Manufacturer Cat# RP01042
Bio-Activity 1.Measured by its binding ability in a functional ELISA. Immobilized Human FGF2 at 0.5 μg/mL (100 μL/well) can bind Human GPC3 with a linear range of 7-20 ng/mL.|2.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.635-2.54 ng/mL, corresponding to a specific activity of 3.94 × 105~1.57 × 106 units/mg.|3.Recombinant Human VEGFA(40 ng/mL, Cat. RP01162) and bFGF(50 ng/mL) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells. After 4 days induction, pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium.|4.The primary neural stem cells were cultured with 20 ng/mL bFGF and observed every 24 h. Results showed that the particle size of the suspended neural stem cells gradually increased.
Alternate Names BFGF, FGF-2, FGFB, HBGF-2,FGF2,FGF-2,FGFB,HBGF-2,Basic FGF, BFGF, fibroblast growth factor 2
Reactivity Human
Gene ID 2247
Swiss Prot P09038-4