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Recombinant Human EGF Protein

Recombinant Human EGF Protein (RP01030-GRP)

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Description

Recombinant Human EGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn 971-Arg 1023) of human EGF (Accession #NP_001954.2) fused with hFc, 6×His tag at the C-terminus.

Specifications

Manufacturer Abclonal Biotechnology
Manufacturer Cat# RP01030
Bio-Activity 1.Measured by its ability to stimulate EGF Receptor autophosphorylation in A431 cells.1-10 ng/mL of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation.|2.Measured by its binding ability in a functional ELISA. Immobilized Human EGFR at 5 μg/mL (100 μL/well) can bind Human EGF with a linear range of 7-25 ng/mL. 3.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.065-0.26 ng/mL, corresponding to a specific activity of 3.84×106-1.54×107units/mg.
Alternate Names EGF,HOMG4,URG
Reactivity Human
Gene ID 1950
Swiss Prot P01133