Recombinant Human DNMT1 Protein
Recombinant Human DNMT1 Protein (RP01868LQ-GRP)
Products
Description
Recombinant Human DNMT1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Pro2-Asp1632) of Human DNMT1 (Accession #NP_001124295.1) fused with a His tag at the C-terminus.
Specifications
| Manufacturer | Abclonal Biotechnology |
|---|---|
| Manufacturer Cat# | RP01868LQ |
| Bio-Activity | The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 µM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 µM SAM in reaction buffer (20 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT) at 37 °C for 1 hr. Then methyltransferase assay was performed using MTase-Glo™ Methyltransferase Assay (Promega, V7601). Enzyme 1-4 represent RP01868LQ, Enzyme 5-8 represent recombinant DNMT1 with a 6×His tag at the N-terminus. (Customer feedback data) |
| Alternate Names | DNMT1, AIM, CXXC9, DNMT,DNA (cytosine-5)-methyltransferase 1, Dnmt1, 2.1.1.37, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI, MCMT |
| Reactivity | Human |
| Gene ID | 1786 |
| Swiss Prot | P26358-2 |