RalA G-LISA GTPase Activation Assay Kit (Colorimetric Based) 96 assays (BK129)

The proprietary RalA G-LISA™ Activation Assaythat is faster, easier and more precise than traditional pull-down methods to measure RalA Activation.

• Specific for RalA
• Colorimetric Based
• Fast Results
• Linear from 0.5 to 5 ng
• Flexible format

General Information

The RalA G-LISA™ kit contains a Ral-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Ral is captured by this protein while inactive GDP-bound Ral is removed following washes. The active RalA bound to the wells is detected with a RalA specific antibody. The degree of RalA activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.

Kit Contents - Enough reagents for 96 assays.

• 96 individual Ral-GTP binding wells
• Anti-RalA monoclonal antibody
• Secondary Antibody - HRP
• RalA control protein
• Ral Binding Buffer
• Cell Lysis Buffer
• Wash buffer
• Antigen Presenting Buffer
• Antibody Dilution Buffer
• HRP Detection and Stop Reagents
• Precision Red™Advanced Protein Assay (Cat. # ADV02)
• Protease Inhibitor Cocktail (Cat. # PIC02)