QuantiChrom™ Phosphate Assay KitQuantiChrom™ Phosphate Assay Kit
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QuantiChrom™ Phosphate Assay Kit

For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism.

• Sensitive and accurate. Linear detection range 0.3 μM (0.0028 mg/dL) to 50 μM (0.47 mg/dL) phosphate in 96-well plate assay.

• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day.

• Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate.

• Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals.

Phosphate (Pi) is one of the most important ion species in nature. Phosphate is present in all biological systems. It is a major constituent in minerals and fertilizers, and is a component of industrial wastewater. Thus accurate determination of phosphate concentration finds numerous applications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring and environmental monitoring. Simple, direct and automation-ready procedures for measuring phosphate concentration in biological and environmental samples are becoming popular. BioAssay Systems’ phosphate assay kit is designed to measure phosphate ion directly in samples without any pretreatment. The improved Malachite Green method utilizes the malachite green dye and molybdate, which forms a stable colored complex specifically with inorganic phosphate. The intensity of the color, measured at 620nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Cat# Size Price Qty Buy
DIPI-500 500 Tests £254.15

Additional Information

Property Value or Rating
Product Size 500 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism.
Method OD620nm (malachite green)
Samples Serum, urine, saliva, sweat, tissue culture, food, environment etc
Species All
Detection Limit 3 μg/dL (0.3 μM)
Storage 4°C
Shelf Life 12 months
References Assay: Phosphate in Rat bone (Pubmed).

2. Abranches, J (2008). CcpA regulates central metabolism and virulence gene expression in Streptococcus mutans. J Bacteriol. 190(7):2340-9. Assay: Phosphate in Bacteria cellular ATPase (Pubmed).

3. Jubeck B et al (2008). Promotion of articular cartilage matrix vesicle mineralization by type I collagen. Arthritis Rheum. 58(9):2809-17. Assay: Phosphate in Human collagen (Pubmed).

4. O’Connor RD et al (2009). Mecp2 deficiency decreases bone formation and reduces bone volume in a rodent model of Rett syndrome. Bone. 45(2):346-56. Assay: Phosphate in Mouse tissue (Pubmed).

5. Dunbar, DR et al (2009). Transcriptional and physiological responses to chronic ACTH treatment by the mouse kidney. Physiol Genomics. 40(3):158-66. Assay: Phosphate in Mouse urine (Pubmed).

6. Ponda MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis. 210(1):57-62. Assay: Phosphate in Mouse tissue (Pubmed).

7. Galitzer H et al (2010). Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease. Kidney Int.77(3):211-8. Assay: Phosphate in Rat serum (Pubmed).

8. Hildebrand, J et al (2009) Functional and energetic characterization of P-gp-mediated doxorubicin transport in rainbow trout (Oncorhynchus mykiss) hepatocytes Comp Biochem Physiol C Toxicol Pharmacol. 149(1):65-72. Assay: Phosphate in Fish Trout hepatocytes (Pubmed).

9. Xie J et al (2010). Comparison of media used to evaluate Rhizobium leguminosarum bivar viciae for phosphate-solubilizing ability. Can J Microbiol. 55(7):910-5. Assay: Phosphate in Plant tissue (Pubmed).

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1. Chung YI et al (2007). Enhanced bone regeneration with BMP-2 loaded functional nanoparticle-hydrogel complex. J Control Release 121(1-2):91-9. Assay: Phosphate in Rat bone (Pubmed).

2. Abranches, J (2008). CcpA regulates central metabolism and virulence gene expression in Streptococcus mutans. J Bacteriol. 190(7):2340-9. Assay: Phosphate in Bacteria cellular ATPase (Pubmed).

3. Jubeck B et al (2008). Promotion of articular cartilage matrix vesicle mineralization by type I collagen. Arthritis Rheum. 58(9):2809-17. Assay: Phosphate in Human collagen (Pubmed).

4. O’Connor RD et al (2009). Mecp2 deficiency decreases bone formation and reduces bone volume in a rodent model of Rett syndrome. Bone. 45(2):346-56. Assay: Phosphate in Mouse tissue (Pubmed).

5. Dunbar, DR et al (2009). Transcriptional and physiological responses to chronic ACTH treatment by the mouse kidney. Physiol Genomics. 40(3):158-66. Assay: Phosphate in Mouse urine (Pubmed).

6. Ponda MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis. 210(1):57-62. Assay: Phosphate in Mouse tissue (Pubmed).

7. Galitzer H et al (2010). Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease. Kidney Int.77(3):211-8. Assay: Phosphate in Rat serum (Pubmed).

8. Hildebrand, J et al (2009) Functional and energetic characterization of P-gp-mediated doxorubicin transport in rainbow trout (Oncorhynchus mykiss) hepatocytes Comp Biochem Physiol C Toxicol Pharmacol. 149(1):65-72. Assay: Phosphate in Fish Trout hepatocytes (Pubmed).

9. Xie J et al (2010). Comparison of media used to evaluate Rhizobium leguminosarum bivar viciae for phosphate-solubilizing ability. Can J Microbiol. 55(7):910-5. Assay: Phosphate in Plant tissue (Pubmed).

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1. Chung YI et al (2007). Enhanced bone regeneration with BMP-2 loaded functional nanoparticle-hydrogel complex. J Control Release 121(1-2):91-9. Assay: Phosphate in Rat bone (Pubmed).

2. Abranches, J (2008). CcpA regulates central metabolism and virulence gene expression in Streptococcus mutans. J Bacteriol. 190(7):2340-9. Assay: Phosphate in Bacteria cellular ATPase (Pubmed).

3. Jubeck B et al (2008). Promotion of articular cartilage matrix vesicle mineralization by type I collagen. Arthritis Rheum. 58(9):2809-17. Assay: Phosphate in Human collagen (Pubmed).

4. O’Connor RD et al (2009). Mecp2 deficiency decreases bone formation and reduces bone volume in a rodent model of Rett syndrome. Bone. 45(2):346-56. Assay: Phosphate in Mouse tissue (Pubmed).

5. Dunbar, DR et al (2009). Transcriptional and physiological responses to chronic ACTH treatment by the mouse kidney. Physiol Genomics. 40(3):158-66. Assay: Phosphate in Mouse urine (Pubmed).

6. Ponda MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis. 210(1):57-62. Assay: Phosphate in Mouse tissue (Pubmed).

7. Galitzer H et al (2010). Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease. Kidney Int.77(3):211-8. Assay: Phosphate in Rat serum (Pubmed).

8. Hildebrand, J et al (2009) Functional and energetic characterization of P-gp-mediated doxorubicin transport in rainbow trout (Oncorhynchus mykiss) hepatocytes Comp Biochem Physiol C Toxicol Pharmacol. 149(1):65-72. Assay: Phosphate in Fish Trout hepatocytes (Pubmed).

9. Xie J et al (2010). Comparison of media used to evaluate Rhizobium leguminosarum bivar viciae for phosphate-solubilizing ability. Can J Microbiol. 55(7):910-5. Assay: Phosphate in Plant tissue (Pubmed).

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