QuantiChrom™ Lactate Dehydrogenase Kit (replacement for DLDH-100)

For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators.

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• High sensitivity and wide linear range. Use 3 μL serum or plasma sample. The detection limit is 2 U/L, linear up to 200 U/L.

• Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of LDH activity within 30 minutes.

• Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

LACTATE DEHYDROGENASE (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.

Cat#	Size	Price	Qty	Buy
D2DH-100	100 Tests	£237.15

Additional Information

Property	Value or Rating
Product Size	100 Tests
Manufacturer	BioAssay Systems
Applications	For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators.
Method	OD565nm
Samples	Serum, plasma etc
Species	All
Detection Limit	2 U/L
Storage	-20°C
Shelf Life	6 months
References	Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 2. Mittal, R et al (2009). Brain damage in newborn rat model of meningitis by Enterobacter sakazakii: a role for outer membrane protein A. Lab Invest 89(3):263-77. Assay: Lactate Dehydrogenase in Rat Serum, brain , intestine (Pubmed). 3. Demers, M et al (2011). Increased efficacy of breast cancer chemotherapy in thrombocytopenic mice. Cancer Res 71(5):1540-9. Assay: Lactate Dehydrogenase in Mouse Tumor homogenate (Pubmed). 4. Moertel, L et al (2008). Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum. Parasitology 135(5):575-83. Assay: Lactate Dehydrogenase in Schistosoma japonicum protein extract (Pubmed). 5. Blanco Garcia, J et al (2009). Physiopathological effects of the NO donor 3-morpholinosydnonimine on rat cortical synaptosomes. Neurochem Res 34(5):931-41. Assay: Lactate Dehydrogenase in Rat Synaptosomal suspension (Pubmed). 6. Yu, CH et al (2009). Inhibitory mechanisms of Agaricus blazei Murill on the growth of prostate cancer in vitro and in vivo. J Nutr Biochem 20(10):753-64. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 7. Kliment, CR et al (2009). Extracellular superoxide dismutase regulates cardiac function and fibrosis. J Mol Cell Cardiol 47(5):730-42. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 8. Cirrik S, Oner G (2009). The effect of heavy muscle activity on renal cytoresistance in rats. Ren Fail 31(8):683-9. Assay: Lactate Dehydrogenase in Rat Blood (Pubmed). 9. Kulik, TB et al (2010). The relationship between oxygen and adenosine in astrocytic cultures. Glia 58(11):1335-44. Assay: Lactate Dehydrogenase in Rat Primary astrocytes, culture medium (Pubmed). 10. Farina, F et al (2011). The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. Toxicol Lett 202(3):209-17. Assay: Lactate Dehydrogenase in Mouse BALF (Pubmed). 11. Pantos, C et al (2011). Acute T3 treatment protects the heart against ischemia-reperfusion injury via TRalpha1 receptor. Mol Cell Biochem 353(1-2):235-41. Assay: Lactate Dehydrogenase in Rat Heart perfudate (Pubmed). 12. Meshulam, T et al (2011). Caveolins/caveolae protect adipocytes from fatty acid-mediated lipotoxicity. J Lipid Res 52(8):1526-32. Assay: Lactate Dehydrogenase in Mouse Cell supernatant (Pubmed). 13. Lu, F et al (2008). The effect of C1 inhibitor on intestinal ischemia and reperfusion injury. Am J Physiol Gastrointest Liver Physiol 295(5):G1042-9. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 14. Kim, YM et al (2009). Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat. Food Chem Toxicol 47(8):2097-102. Assay: Lactate Dehydrogenase in Rat Serum (Pubmed). 15. van Vlerken, LE et al (2010). Augmentation of therapeutic efficacy in drug-resistant tumor models using ceramide coadministration in temporal-controlled polymer-blend nanoparticle delivery systems. AAPS J 12(2):171-80. Assay: Lactate Dehydrogenase in Mouse Plasma (Pubmed). 16. Tanaka, T et al (2010). In vivo evaluation of safety of nanoporous silicon carriers following single and multiple dose intravenous administrations in mice. Int J Pharm 402(1-2):190-7. Assay: Lactate Dehydrogenase in Mouse Plasma (Pubmed). 17. Uchida, S et al (2011). Combination of chondroitin sulfate and polyplex micelles from Poly(ethylene glycol)-poly{N’-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} block copolymer for prolonged in vivo gene transfection with reduced toxicity. J Control Release . Assay: Lactate Dehydrogenase in Mouse BALF (Pubmed). " title=" 1. Marques, R et al (2008). B lymphocyte activation by coinfection prevents immune control of friend virus infection. J Immunol 181(5):3432-40. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 2. Mittal, R et al (2009). Brain damage in newborn rat model of meningitis by Enterobacter sakazakii: a role for outer membrane protein A. Lab Invest 89(3):263-77. Assay: Lactate Dehydrogenase in Rat Serum, brain , intestine (Pubmed). 3. Demers, M et al (2011). Increased efficacy of breast cancer chemotherapy in thrombocytopenic mice. Cancer Res 71(5):1540-9. Assay: Lactate Dehydrogenase in Mouse Tumor homogenate (Pubmed). 4. Moertel, L et al (2008). Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum. Parasitology 135(5):575-83. Assay: Lactate Dehydrogenase in Schistosoma japonicum protein extract (Pubmed). 5. Blanco Garcia, J et al (2009). Physiopathological effects of the NO donor 3-morpholinosydnonimine on rat cortical synaptosomes. Neurochem Res 34(5):931-41. Assay: Lactate Dehydrogenase in Rat Synaptosomal suspension (Pubmed). 6. Yu, CH et al (2009). Inhibitory mechanisms of Agaricus blazei Murill on the growth of prostate cancer in vitro and in vivo. J Nutr Biochem 20(10):753-64. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 7. Kliment, CR et al (2009). Extracellular superoxide dismutase regulates cardiac function and fibrosis. J Mol Cell Cardiol 47(5):730-42. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 8. Cirrik S, Oner G (2009). The effect of heavy muscle activity on renal cytoresistance in rats. Ren Fail 31(8):683-9. Assay: Lactate Dehydrogenase in Rat Blood (Pubmed). 9. Kulik, TB et al (2010). The relationship between oxygen and adenosine in astrocytic cultures. Glia 58(11):1335-44. Assay: Lactate Dehydrogenase in Rat Primary astrocytes, culture medium (Pubmed). 10. Farina, F et al (2011). The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. Toxicol Lett 202(3):209-17. Assay: Lactate Dehydrogenase in Mouse BALF (Pubmed). 11. Pantos, C et al (2011). Acute T3 treatment protects the heart against ischemia-reperfusion injury via TRalpha1 receptor. Mol Cell Biochem 353(1-2):235-41. Assay: Lactate Dehydrogenase in Rat Heart perfudate (Pubmed). 12. Meshulam, T et al (2011). Caveolins/caveolae protect adipocytes from fatty acid-mediated lipotoxicity. J Lipid Res 52(8):1526-32. Assay: Lactate Dehydrogenase in Mouse Cell supernatant (Pubmed). 13. Lu, F et al (2008). The effect of C1 inhibitor on intestinal ischemia and reperfusion injury. Am J Physiol Gastrointest Liver Physiol 295(5):G1042-9. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 14. Kim, YM et al (2009). Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat. Food Chem Toxicol 47(8):2097-102. Assay: Lactate Dehydrogenase in Rat Serum (Pubmed). 15. van Vlerken, LE et al (2010). Augmentation of therapeutic efficacy in drug-resistant tumor models using ceramide coadministration in temporal-controlled polymer-blend nanoparticle delivery systems. AAPS J 12(2):171-80. Assay: Lactate Dehydrogenase in Mouse Plasma (Pubmed). 16. Tanaka, T et al (2010). In vivo evaluation of safety of nanoporous silicon carriers following single and multiple dose intravenous administrations in mice. Int J Pharm 402(1-2):190-7. Assay: Lactate Dehydrogenase in Mouse Plasma (Pubmed). 17. Uchida, S et al (2011). Combination of chondroitin sulfate and polyplex micelles from Poly(ethylene glycol)-poly{N’-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} block copolymer for prolonged in vivo gene transfection with reduced toxicity. J Control Release . Assay: Lactate Dehydrogenase in Mouse BALF (Pubmed). " target="_blank"> 1. Marques, R et al (2008). B lymphocyte activation by coinfection prevents immune control of friend virus infection. J Immunol 181(5):3432-40. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 2. Mittal, R et al (2009). Brain damage in newborn rat model of meningitis by Enterobacter sakazakii: a role for outer membrane protein A. Lab Invest 89(3):263-77. Assay: Lactate Dehydrogenase in Rat Serum, brain , intestine (Pubmed). 3. Demers, M et al (2011). Increased efficacy of breast cancer chemotherapy in thrombocytopenic mice. Cancer Res 71(5):1540-9. Assay: Lactate Dehydrogenase in Mouse Tumor homogenate (Pubmed). 4. Moertel, L et al (2008). Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum. Parasitology 135(5):575-83. Assay: Lactate Dehydrogenase in Schistosoma japonicum protein extract (Pubmed). 5. Blanco Garcia, J et al (2009). Physiopathological effects of the NO donor 3-morpholinosydnonimine on rat cortical synaptosomes. Neurochem Res 34(5):931-41. Assay: Lactate Dehydrogenase in Rat Synaptosomal suspension (Pubmed). 6. Yu, CH et al (2009). Inhibitory mechanisms of Agaricus blazei Murill on the growth of prostate cancer in vitro and in vivo. J Nutr Biochem 20(10):753-64. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 7. Kliment, CR et al (2009). Extracellular superoxide dismutase regulates cardiac function and fibrosis. J Mol Cell Cardiol 47(5):730-42. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 8. Cirrik S, Oner G (2009). The effect of heavy muscle activity on renal cytoresistance in rats. Ren Fail 31(8):683-9. Assay: Lactate Dehydrogenase in Rat Blood (Pubmed). 9. Kulik, TB et al (2010). The relationship between oxygen and adenosine in astrocytic cultures. Glia 58(11):1335-44. Assay: Lactate Dehydrogenase in Rat Primary astrocytes, culture medium (Pubmed). 10. Farina, F et al (2011). The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. Toxicol Lett 202(3):209-17. Assay: Lactate Dehydrogenase in Mouse BALF (Pubmed). 11. Pantos, C et al (2011). Acute T3 treatment protects the heart against ischemia-reperfusion injury via TRalpha1 receptor. Mol Cell Biochem 353(1-2):235-41. Assay: Lactate Dehydrogenase in Rat Heart perfudate (Pubmed). 12. Meshulam, T et al (2011). Caveolins/caveolae protect adipocytes from fatty acid-mediated lipotoxicity. J Lipid Res 52(8):1526-32. Assay: Lactate Dehydrogenase in Mouse Cell supernatant (Pubmed). 13. Lu, F et al (2008). The effect of C1 inhibitor on intestinal ischemia and reperfusion injury. Am J Physiol Gastrointest Liver Physiol 295(5):G1042-9. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed). 14. Kim, YM et al (2009). Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat. Food Chem Toxicol 47(8):2097-102. Assay: Lactate Dehydrogenase in Rat Serum (Pubmed). 15. van Vlerken, LE et al (2010). Augmentation of therapeutic efficacy in drug-resistant tumor models using ceramide coadministration in temporal-controlled polymer-blend nanoparticle delivery systems. AAPS J 12(2):171-80. Assay: Lactate Dehydrogenase in Mouse Plasma (Pubmed). 16. Tanaka, T et al (2010). In vivo evaluation of safety of nanoporous silicon carriers following single and multiple dose intravenous administrations in mice. Int J Pharm 402(1-2):190-7. Assay: Lactate Dehydrogenase in Mouse Plasma (Pubmed). 17. Uchida, S et al (2011). Combination of chondroitin sulfate and polyplex micelles from Poly(ethylene glycol)-poly{N’-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} block copolymer for prolonged in vivo gene transfection with reduced toxicity. J Control Release . Assay: Lactate Dehydrogenase in Mouse BALF (Pubmed).

Related Documents

1. Marques, R et al (2008). B lymphocyte activation by coinfection prevents immune control of friend virus infection. J Immunol 181(5):3432-40. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed).

2. Mittal, R et al (2009). Brain damage in newborn rat model of meningitis by Enterobacter sakazakii: a role for outer membrane protein A. Lab Invest 89(3):263-77. Assay: Lactate Dehydrogenase in Rat Serum, brain , intestine (Pubmed).

3. Demers, M et al (2011). Increased efficacy of breast cancer chemotherapy in thrombocytopenic mice. Cancer Res 71(5):1540-9. Assay: Lactate Dehydrogenase in Mouse Tumor homogenate (Pubmed).

4. Moertel, L et al (2008). Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum. Parasitology 135(5):575-83. Assay: Lactate Dehydrogenase in Schistosoma japonicum protein extract (Pubmed).

5. Blanco Garcia, J et al (2009). Physiopathological effects of the NO donor 3-morpholinosydnonimine on rat cortical synaptosomes. Neurochem Res 34(5):931-41. Assay: Lactate Dehydrogenase in Rat Synaptosomal suspension (Pubmed).

6. Yu, CH et al (2009). Inhibitory mechanisms of Agaricus blazei Murill on the growth of prostate cancer in vitro and in vivo. J Nutr Biochem 20(10):753-64. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed).

7. Kliment, CR et al (2009). Extracellular superoxide dismutase regulates cardiac function and fibrosis. J Mol Cell Cardiol 47(5):730-42. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed).

8. Cirrik S, Oner G (2009). The effect of heavy muscle activity on renal cytoresistance in rats. Ren Fail 31(8):683-9. Assay: Lactate Dehydrogenase in Rat Blood (Pubmed).

9. Kulik, TB et al (2010). The relationship between oxygen and adenosine in astrocytic cultures. Glia 58(11):1335-44. Assay: Lactate Dehydrogenase in Rat Primary astrocytes, culture medium (Pubmed).

10. Farina, F et al (2011). The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. Toxicol Lett 202(3):209-17. Assay: Lactate Dehydrogenase in Mouse BALF (Pubmed).

11. Pantos, C et al (2011). Acute T3 treatment protects the heart against ischemia-reperfusion injury via TRalpha1 receptor. Mol Cell Biochem 353(1-2):235-41. Assay: Lactate Dehydrogenase in Rat Heart perfudate (Pubmed).

12. Meshulam, T et al (2011). Caveolins/caveolae protect adipocytes from fatty acid-mediated lipotoxicity. J Lipid Res 52(8):1526-32. Assay: Lactate Dehydrogenase in Mouse Cell supernatant (Pubmed).

13. Lu, F et al (2008). The effect of C1 inhibitor on intestinal ischemia and reperfusion injury. Am J Physiol Gastrointest Liver Physiol 295(5):G1042-9. Assay: Lactate Dehydrogenase in Mouse Serum (Pubmed).

14. Kim, YM et al (2009). Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat. Food Chem Toxicol 47(8):2097-102. Assay: Lactate Dehydrogenase in Rat Serum (Pubmed).

15. van Vlerken, LE et al (2010). Augmentation of therapeutic efficacy in drug-resistant tumor models using ceramide coadministration in temporal-controlled polymer-blend nanoparticle delivery systems. AAPS J 12(2):171-80. Assay: Lactate Dehydrogenase in Mouse Plasma (Pubmed).

16. Tanaka, T et al (2010). In vivo evaluation of safety of nanoporous silicon carriers following single and multiple dose intravenous administrations in mice. Int J Pharm 402(1-2):190-7. Assay: Lactate Dehydrogenase in Mouse Plasma (Pubmed).

17. Uchida, S et al (2011). Combination of chondroitin sulfate and polyplex micelles from Poly(ethylene glycol)-poly{N’-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} block copolymer for prolonged in vivo gene transfection with reduced toxicity. J Control Release . Assay: Lactate Dehydrogenase in Mouse BALF (Pubmed).

QuantiChrom™ Lactate Dehydrogenase Kit (replacement for DLDH-100)

Additional Information

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