QuantiChrom™ β-Glucosidase Assay KitQuantiChrom™ β-Glucosidase Assay Kit
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QuantiChrom™ β-Glucosidase Assay Kit

For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism.

• High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.

• Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes.

• Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

β-GLUCOSIDASE is a glucosidase enzyme which acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). β-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a β-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a β-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher’s disease and Parkinson’s disease. Simple, direct and automation-ready procedures for measuring β-glucosidase activity are becoming popular in Research and Drug Discovery. BioAssay Systems’ QuantiChrom™ β-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

Cat# Size Price Qty Buy
DBGD-100 100 Tests £322.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism.
Method OD405nm
Samples Biological
Species All
Detection Limit 2 U/L
Storage -20°C
Shelf Life 6 months
References Assay: ?-Glucosidase in Human saliva (Pubmed).

2. Renchinkhand, G et al (2015). Identification of ?-Glucosidase Activity of Enterococcus Faecalis CRNB-A3 in Airag and Its Potential to Convert Ginsenoside Rb1 from Panax Ginseng. Journal of Food Biochemistry 40(1): 120-129. Bacteria in Enterococcus faecalis Assay.

3. Liu, Z et al (2014). Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach. Biotechnology Letters 36(11): 2169-2176. Assay: ?-Glucosidase in Human saliva (Cells in Mammals) (Pubmed). 

4. Moon, JY et al (2015). Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines. Animal Cells and Systems 19(5): 295-304. Cells in Human Assay. 

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1. Mallery, SR et al (2011). Effects of human oral mucosal tissue, saliva, and oral microflora on intraoral metabolism and bioactivation of black raspberry anthocyanins. Cancer Prev Res (Phila). 4(8):1209-21. Assay: ?-Glucosidase in Human saliva (Pubmed).

2. Renchinkhand, G et al (2015). Identification of ?-Glucosidase Activity of Enterococcus Faecalis CRNB-A3 in Airag and Its Potential to Convert Ginsenoside Rb1 from Panax Ginseng. Journal of Food Biochemistry 40(1): 120-129. Bacteria in Enterococcus faecalis Assay.

3. Liu, Z et al (2014). Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach. Biotechnology Letters 36(11): 2169-2176. Assay: ?-Glucosidase in Human saliva (Cells in Mammals) (Pubmed). 

4. Moon, JY et al (2015). Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines. Animal Cells and Systems 19(5): 295-304. Cells in Human Assay. 

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1. Mallery, SR et al (2011). Effects of human oral mucosal tissue, saliva, and oral microflora on intraoral metabolism and bioactivation of black raspberry anthocyanins. Cancer Prev Res (Phila). 4(8):1209-21. Assay: ?-Glucosidase in Human saliva (Pubmed).

2. Renchinkhand, G et al (2015). Identification of ?-Glucosidase Activity of Enterococcus Faecalis CRNB-A3 in Airag and Its Potential to Convert Ginsenoside Rb1 from Panax Ginseng. Journal of Food Biochemistry 40(1): 120-129. Bacteria in Enterococcus faecalis Assay.

3. Liu, Z et al (2014). Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach. Biotechnology Letters 36(11): 2169-2176. Assay: ?-Glucosidase in Human saliva (Cells in Mammals) (Pubmed). 

4. Moon, JY et al (2015). Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines. Animal Cells and Systems 19(5): 295-304. Cells in Human Assay. 

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