QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit
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QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit

For evaluation and high-throughput screen (HTS) of glucose-6-phosphate dehydrogenase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed. Method: OD565nm. Samples: Glucose-6-phosphate dehydrogenase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

•  Safe. Non-radioactive assay.

•  High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system.

•  Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed.

GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics. BioAssay Systems' non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.

Cat# Size Price Qty Buy
IGPDH-100 100 Tests £364.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications HTS for inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.
Method OD565nm
Samples Glucose-6-phosphate dehydrogenase
Species All
Storage -20 °C
Shelf Life 12 months

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