QuantiChrom™ BCG Albumin Assay KitQuantiChrom™ BCG Albumin Assay Kit
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QuantiChrom™ BCG Albumin Assay Kit

For quantitative determination of albumin.

• Sensitive and accurate. Use as little as 5 μL samples. Detection range 0.01 g/dL (1.5 mM) to 5 g/dL (750 mM) albumin in 96-well plate assay.

• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

• Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. 

• No interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.

Albumin is the most abundant plasma protein in human. It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure, binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium and magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition and protein-losing enteropathies. High levels are associated with dehydration. Simple, direct and automation-ready procedures for measuring albumin concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems’ BCG albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol green that forms a colored complex specifically with albumin. The intensity of the color, measured at 620nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Cat# Size Price Qty Buy
DIAG-250 250 Tests £245.65

Additional Information

Property Value or Rating
Product Size 250 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of albumin.
Method OD620nm (BCG)
Samples Serum, plasma, urine, biological preparations
Species All
Detection Limit 0.01 g/dL
Storage 4, -20°C
Shelf Life 12 months
References Assay: Albumin in Human albuminuria (Pubmed).

2. Lee, RH et al (2006) Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD_scid mice. PNAS 103 (46): 17438?17443. Assay: Albumin in Mouse urine (Pubmed).

3. Helsby, NA et al (2008). CYP2C19 pharmacogenetics in advanced cancer: compromised function independent of genotype. Br J Cancer. 99(8):1251-5. Assay: Albumin in Human serum (Pubmed).

4. Doi, M et al (2011). Association between calcium in cord blood and newborn size in Bangladesh. Br J Nutr. 2011 Jun 1:1-10. Assay: Albumin in Human blood (Pubmed).

5. Zhang, Q et al (2006). Serum and mucosal antibody responses to pneumococcal protein antigens in children: relationships with carriage status. Eur J Immunol.36(1):46-57. Assay: Albumin in Human saliva, serum (Pubmed).

6. Cosgrove, D et al (2008). Integrin alpha1?1 Regulates Matrix Metalloproteinases via P38 Mitogen-Activated Protein Kinase in Mesangial Cells. Implications for Alport Syndrome. Am. J. Pathology 172:761-773. Assay: Albumin in Mouse urine (Pubmed).

7. Zhang, G et al (2011). Nicotinic acetylcholine receptor ?1 promotes calpain-1 activation and macrophage inflammation in hypercholesterolemic nephropathy. Lab Invest. 91(1):106-23. Assay: Albumin in Mouse urine (Pubmed).

8. Euhus, DM et al (2011). Tamoxifen Downregulates Ets-oncogene Family Members ETV4 and ETV5 in Benign Breast Tissue: Implications for Durable Risk Reduction. Cancer Prev Res (Phila). 2011 Jul 21. Assay: Albumin in Human blood (Pubmed).

9. Wang, S et al (2006). Renal bone morphogenetic protein-7 protects against diabetic nephropathy. J Am Soc Nephrol. 17(9):2504-12. Assay: Albumin in Mouse serum (Pubmed).

10. Rebecca, R (2006). Associations of histories of depression and PMDD diagnosis with allopregnanolone concentrations following the oral administration of micronized progesterone sychoneuroendocrinology 31(10):1208-1219. Assay: Albumin in Human serum (Pubmed).

11. Sullivan, JP et al (2008). Convection and hemoglobin-based oxygen carrier enhanced oxygen transport in a hepatic hollow fiber bioreactor. Artif Cells Blood Substit Immobil Biotechnol.36(4):386-402. Assay: Albumin in Human hepatocyte (Pubmed).

12. Osborn, TM et al (2008). Decreased levels of the gelsolin plasma isoform in patients with rheumatoid arthritis. Arthritis Res Ther. 10(5):R117. Assay: Albumin in Human plasma, synovial fluid (Pubmed).

13. Roberts, DJ et al (2009). Effect of acute inflammatory brain injury on accumulation of morphine and morphine 3- and 6-glucuronide in the human brain. Crit Care Med. 37(10):2767-74. Assay: Albumin in Human plasma, cerebrospinal fluid (Pubmed).

14. Irani, K et al (2010). Mechanical dissociation of swine liver to produce organoid units for tissue engineering and in vitro disease modeling. Artif Organs. 34(1):75-8. Assay: Albumin in Pig hepatocyte (Pubmed).

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1. Kain, R et al (2008). Molecular mimicry in pauci-immune focal necrotizing glomerulonephritis. Nat Med. 14(10):1088-96. Assay: Albumin in Human albuminuria (Pubmed).

2. Lee, RH et al (2006) Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD_scid mice. PNAS 103 (46): 17438?17443. Assay: Albumin in Mouse urine (Pubmed).

3. Helsby, NA et al (2008). CYP2C19 pharmacogenetics in advanced cancer: compromised function independent of genotype. Br J Cancer. 99(8):1251-5. Assay: Albumin in Human serum (Pubmed).

4. Doi, M et al (2011). Association between calcium in cord blood and newborn size in Bangladesh. Br J Nutr. 2011 Jun 1:1-10. Assay: Albumin in Human blood (Pubmed).

5. Zhang, Q et al (2006). Serum and mucosal antibody responses to pneumococcal protein antigens in children: relationships with carriage status. Eur J Immunol.36(1):46-57. Assay: Albumin in Human saliva, serum (Pubmed).

6. Cosgrove, D et al (2008). Integrin alpha1?1 Regulates Matrix Metalloproteinases via P38 Mitogen-Activated Protein Kinase in Mesangial Cells. Implications for Alport Syndrome. Am. J. Pathology 172:761-773. Assay: Albumin in Mouse urine (Pubmed).

7. Zhang, G et al (2011). Nicotinic acetylcholine receptor ?1 promotes calpain-1 activation and macrophage inflammation in hypercholesterolemic nephropathy. Lab Invest. 91(1):106-23. Assay: Albumin in Mouse urine (Pubmed).

8. Euhus, DM et al (2011). Tamoxifen Downregulates Ets-oncogene Family Members ETV4 and ETV5 in Benign Breast Tissue: Implications for Durable Risk Reduction. Cancer Prev Res (Phila). 2011 Jul 21. Assay: Albumin in Human blood (Pubmed).

9. Wang, S et al (2006). Renal bone morphogenetic protein-7 protects against diabetic nephropathy. J Am Soc Nephrol. 17(9):2504-12. Assay: Albumin in Mouse serum (Pubmed).

10. Rebecca, R (2006). Associations of histories of depression and PMDD diagnosis with allopregnanolone concentrations following the oral administration of micronized progesterone sychoneuroendocrinology 31(10):1208-1219. Assay: Albumin in Human serum (Pubmed).

11. Sullivan, JP et al (2008). Convection and hemoglobin-based oxygen carrier enhanced oxygen transport in a hepatic hollow fiber bioreactor. Artif Cells Blood Substit Immobil Biotechnol.36(4):386-402. Assay: Albumin in Human hepatocyte (Pubmed).

12. Osborn, TM et al (2008). Decreased levels of the gelsolin plasma isoform in patients with rheumatoid arthritis. Arthritis Res Ther. 10(5):R117. Assay: Albumin in Human plasma, synovial fluid (Pubmed).

13. Roberts, DJ et al (2009). Effect of acute inflammatory brain injury on accumulation of morphine and morphine 3- and 6-glucuronide in the human brain. Crit Care Med. 37(10):2767-74. Assay: Albumin in Human plasma, cerebrospinal fluid (Pubmed).

14. Irani, K et al (2010). Mechanical dissociation of swine liver to produce organoid units for tissue engineering and in vitro disease modeling. Artif Organs. 34(1):75-8. Assay: Albumin in Pig hepatocyte (Pubmed).

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1. Kain, R et al (2008). Molecular mimicry in pauci-immune focal necrotizing glomerulonephritis. Nat Med. 14(10):1088-96. Assay: Albumin in Human albuminuria (Pubmed).

2. Lee, RH et al (2006) Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD_scid mice. PNAS 103 (46): 17438?17443. Assay: Albumin in Mouse urine (Pubmed).

3. Helsby, NA et al (2008). CYP2C19 pharmacogenetics in advanced cancer: compromised function independent of genotype. Br J Cancer. 99(8):1251-5. Assay: Albumin in Human serum (Pubmed).

4. Doi, M et al (2011). Association between calcium in cord blood and newborn size in Bangladesh. Br J Nutr. 2011 Jun 1:1-10. Assay: Albumin in Human blood (Pubmed).

5. Zhang, Q et al (2006). Serum and mucosal antibody responses to pneumococcal protein antigens in children: relationships with carriage status. Eur J Immunol.36(1):46-57. Assay: Albumin in Human saliva, serum (Pubmed).

6. Cosgrove, D et al (2008). Integrin alpha1?1 Regulates Matrix Metalloproteinases via P38 Mitogen-Activated Protein Kinase in Mesangial Cells. Implications for Alport Syndrome. Am. J. Pathology 172:761-773. Assay: Albumin in Mouse urine (Pubmed).

7. Zhang, G et al (2011). Nicotinic acetylcholine receptor ?1 promotes calpain-1 activation and macrophage inflammation in hypercholesterolemic nephropathy. Lab Invest. 91(1):106-23. Assay: Albumin in Mouse urine (Pubmed).

8. Euhus, DM et al (2011). Tamoxifen Downregulates Ets-oncogene Family Members ETV4 and ETV5 in Benign Breast Tissue: Implications for Durable Risk Reduction. Cancer Prev Res (Phila). 2011 Jul 21. Assay: Albumin in Human blood (Pubmed).

9. Wang, S et al (2006). Renal bone morphogenetic protein-7 protects against diabetic nephropathy. J Am Soc Nephrol. 17(9):2504-12. Assay: Albumin in Mouse serum (Pubmed).

10. Rebecca, R (2006). Associations of histories of depression and PMDD diagnosis with allopregnanolone concentrations following the oral administration of micronized progesterone sychoneuroendocrinology 31(10):1208-1219. Assay: Albumin in Human serum (Pubmed).

11. Sullivan, JP et al (2008). Convection and hemoglobin-based oxygen carrier enhanced oxygen transport in a hepatic hollow fiber bioreactor. Artif Cells Blood Substit Immobil Biotechnol.36(4):386-402. Assay: Albumin in Human hepatocyte (Pubmed).

12. Osborn, TM et al (2008). Decreased levels of the gelsolin plasma isoform in patients with rheumatoid arthritis. Arthritis Res Ther. 10(5):R117. Assay: Albumin in Human plasma, synovial fluid (Pubmed).

13. Roberts, DJ et al (2009). Effect of acute inflammatory brain injury on accumulation of morphine and morphine 3- and 6-glucuronide in the human brain. Crit Care Med. 37(10):2767-74. Assay: Albumin in Human plasma, cerebrospinal fluid (Pubmed).

14. Irani, K et al (2010). Mechanical dissociation of swine liver to produce organoid units for tissue engineering and in vitro disease modeling. Artif Organs. 34(1):75-8. Assay: Albumin in Pig hepatocyte (Pubmed).

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