QuantiChrom™ Alcohol dehydrogenase Assay KitQuantiChrom™ Alcohol dehydrogenase Assay Kit
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QuantiChrom™ Alcohol dehydrogenase Assay Kit

For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.

• Fast and sensitive. Linear detection range (20 μL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. 

• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation. BioAssay Systems’ non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Cat# Size Price Qty Buy
DADH-100 100 Tests £262.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.
Method OD565nm
Samples Biological (e.g. plasma, serum, urine, tissue and culture media)
Species All
Detection Limit 0.4 U/L
Storage -20°C
Shelf Life 6 months

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