Purine Nucleoside Phosphorylase (PNP) (ELP03)

Product Uses

• Measurement of phosphate in buffers or serum.
• Identification/characterization of ATPase proteins.
• Measurement of enzymatic parameters of ATPases and GTPases. 

Materials

The purine nucleoside phosphorylase protein has been produced in a bacterial expression system.  The recombinant protein (aa1-283) contains a 6xHIS-tag at the amino terminus and has a combined calculated molecular weight of approximately 32 kDa.  The protein has been determined to be biologically active in a microtubule-activated phosphate transfer assay (see below).  The protein is supplied as a white lyophilized powder. 

Storage and Resonstitution

Shipped at ambient temperature. The recommended storage conditions for the lyophilized material is 4°C and <10% humidity.  Under these conditions the protein is stable for 1 year.   

Briefly centrifuge to collect the protein at the bottom of the tube. The protein should be reconstituted to 2 mg/ml with distilled water and stored at 4°C where it will be stable for 6 months. For working concentrations dilute 100 fold into the reaction mixture. 

Purity

Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on an SDS gradient gel.