Product Uses
- Measurement of phosphate in buffers or serum.
- Identification/characterization of ATPase proteins.
- Measurement of enzymatic parameters of ATPases and GTPases.
Materials
The purine nucleoside phosphorylase protein has been produced in a bacterial expression system. The recombinant protein (aa1-283) contains a 6xHIS-tag at the amino terminus and has a combined calculated molecular weight of approximately 32 kDa. The protein has been determined to be biologically active in a microtubule-activated phosphate transfer assay (see below). The protein is supplied as a white lyophilized powder.
Storage and Resonstitution
Shipped at ambient temperature. The recommended storage conditions for the lyophilized material is 4°C and <10% humidity. Under these conditions the protein is stable for 1 year.
Briefly centrifuge to collect the protein at the bottom of the tube. The protein should be reconstituted to 2 mg/ml with distilled water and stored at 4°C where it will be stable for 6 months. For working concentrations dilute 100 fold into the reaction mixture.
Purity
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on an SDS gradient gel.
