Profilin 1 protein - Untagged - human recombinant (PR02-GRP)

Product Uses Include

• Actin binding studies
• Actin polymerization studies

Material

Profilin protein has been expressed in E.coli and purified via a 6xHis tag that is later cleaved by protease digestion. Thus the protein does not contian a 6xHis or other tag. The protein is supplied as a lyophilized powder. When resuspended in nanopure water, the profilin will be in the following buffer:  10 mM Tris pH 8.0, 1 mM EDTA, 1 mM DTT, 5% (w/v) sucrose and 1% (w/v) dextran.

The lyophilized protein is stable at 4°C desiccated (<10% humidity) for up to 1 year.

Purity

Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Purity is >95% (see figure 1).