ProbeMaster Lyo UDG, 5x (L0514)

ProbeMaster® Lyo UDG is a lyophilized reaction mixture containing all the necessary components for PCR. To reconstitute the mixture into a liquid form, simply add the specified amount of water. The composition of the mixture includes Hot-start polymerase and is optimized to achieve optimal amplification processivity and specificity. Uracil-DNA glycosylase eliminates contamination from amplicons generated in prior reactions, preventing false-positive results. The ProbeMaster® Lyo UDG mixture is suitable for both quantitative PCR and DNA amplification followed by electrophoresis detection. For qPCR reactions, fluorescence detection should use a DNA probe labeled with a fluorophore and quencher (hydrolyzable probes, «molecular beacons,» «scorpion» primers) or two probes labeled with fluorophores that form a FRET pair. In addition to DNA probes, the intercalating dye dsGreen can be used for fluorescence detection. One tube of lyophilized mixture, after dilution in 450 μL of water, is sufficient for 100 reactions of 25 μL each.