Product uses include measuring protein in
- Cell extracts
- Serum samples
- Medium to high protein concentration solutions
- Protein requiring GLP and higher standards
- Purified proteins and antibodies
Material
The Precision Red Advanced Protein Assay Reagent is designed to optimize the speed and accuracy of protein measurement. The reagent combines the useful properties of low protein to protein variance (See Fig 1) an d a wide protein concentration for a robust assay. The assay can measure accurately protein concentrations ranging from 0.25 to 50 mg/ml and is detergent compatible. A simple one step procedure results in a red to blue color change within 1 min, which can be recognized by measuring absorbance at 600 nm.
This reagent is so reproducible with a wide range of proteins and protein concentrations that it is employed to accurately determine the concentration in all of Cytoskeleton, Inc's protein products.
Method
The Precision Red Advanced Protein Assay makes it very easy and quick to measure prottein concentrations. A general method looks as follows:
- Mix protein solution with ADV02 reagent (e.g. 10 µl sample with 1.0 ml ADV02)
- Incubate 1 min
- Read absorbance at 600 nm
Detergent and chemical compatibility
The Precision Red Advanced Protein Assay is a highly stable assay and it tolerates a wide range of reagents.
Using the Precision Red Advanced Protein Assay in cell extracts
Since the Precision Red Advanced Protein Assay is highly compatible with non-ionic detergents, this reagent is ideal for measuring protein concentrations in cell and tissue extracts. Furthermore, since protein concentration can be measured after only 1 min of incubation with the reagent, it allows the user to proceed with the experiment quicker than any other protein assay, something that can be of big importance when working with labile cell extracts.
Cytoskeleton, Inc. recommends using ADV02 for all our kits and assays that involve cell or tissue lysis. In the G-LISA small G-protein activation kits, we have even included this reagent to ensure that the user gets the most accurate and quickest possible measurement of the protein concentration in the cell lysates generated.
