Pre-formed actin filaments (rabbit skeletal muscle)Pre-formed actin filaments (rabbit skeletal muscle)
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Pre-formed actin filaments (rabbit skeletal muscle)

Product uses

  • Substrate for activation of myosin ATPases 
  • Substrate for discovery and characterization of actin binding proteins (ABPs)
  • Determination of IC50s for myosin inhibitors
  • Used in Cytoskeleton, Inc’s ATPase assays (Cat. # BK051 and BK054) 
  • Ideal for HTS applications

Material
Pre-formed actin filaments (PAFs) are supplied as a lyophilized powder. PAFs have been prepared from rabbit skeletal muscle actin protein that is greater than 99% pure (Cat. # AKL99). These stringently quality controlled filaments provide highly reliable and
  reproducible results in assays that require actin filament substrates. The average filament length in this product is 8 µm. Using these PAFs in assays depending on filaments gives very reproducible results. In an actin filament activated myosin ATPase assay, the CV between samples (Vmax) was less than 5% and the CV of Vmax between assays using different batches of AKF99 was less than 8%.

Purity
The actin protein used to make pre-formed actin filaments is >99% pure. See Cat. # AKL99.

Biological Activity
PAFs serve as a substrate for myosin motor proteins. Myosin motor proteins orchestrate a wide range of kinetic events within a cell. They have been shown to move cargoes such as vesicles along actin filament tracks. Myosins operate by utilizing the energy of 
ATP to hydrolysis, an activity that is greatly enhanced in the presence of PAFs. A PAF activated Enzyme Linked Inorganic Phosphate ATPase (ELIPA™) assay (Cat. # BK051 and BK054) can therefore be used as a test for the biological activity of AKF99 (Fig. 1). The motor protein used in this assay is myosin motor protein (Myosin II: Cat. # MY02 or MY03). AKF99 is also an excellent substrate for detecting actin binding proteins.

Cat# Size Price Qty Buy
AKF99-A 1 x 1 mg
£161.46
AKF99-B 5 x 1 mg
£574.86

Additional Information

Property Value or Rating
Manufacturer Cytoskeleton, Inc
Storage 4°C

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