PNGase F, recombinant lyophilized (36405.01)

Concentration after reconstitution: 1000 u/µl (2.0 mg/ml) in 50 µl H2O dest.
PNGase F is a mutant recombinant glycosidase from Flavobacterium meningosepticum and expressed and purified from E. coli. The enzyme catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins.
The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources of PNGase F:

• No need for refrigerated transport, storage at room temperature
• Does not need a denaturing step
• Works on native glycoproteins and serum glycoproteins in only minutes at room temperature
• Digestion leads to a more complete glycan release as compared to other commercially-available enzymes
• Especially designed and tested for mass spectrometry imaging of tissue samples