Nitro Blue tetrazolium chloride (NBT) analytical grade (30550-GRP)

A ditetrazolium compound for demonstrating succinic dehydrogenase activity (1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). Used in conjuction with BCIP (cat. no. 15247) for detection of alkaline phosphatase.Stock solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C.Staining solution for Western Blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5)