Myosin II protein - rabbit skeletal muscle (MY02-GRP)

Product Uses Include

• Measurement of F-actin activated myosin ATPase activity
• Identification/characterization of proteins or small molecules that affect myosin II ATPase activity
• Identification/characterization of proteins or small molecules that affect the myosin II - F-actin interaction
• Control myosin ATPase activity in drug discovery screening projects

Material
Myosin II protein has been purified from rabbit skeletal muscle. The full length myosin II protein has been purified with its essential light chains (ELC) and regulatory light chains (RLC), see Figure 1 and 2. Myosin II has been determined to be biologically active in an F-actin activated ATPase assay. Rabbit skeletal muscle myosin II is not recommended for use in motility assays. Rabbit myosin II protein is supplied as a white lyophilized powder. When reconstituted in nanopure water, the protein will be in the following buffer: 100 mM Imidizole pH 7.5, 2.4 M KCl, 0.2 mM DTT, 5% sucrose and 1% dextran. The lyophilized protein is stable at 4°C desiccated (<10% humidity) for 1 year. 

Purity
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% gradient polyacrylamide gel. Myosin protein is 95% pure.