MsrB2 Protein 6xHis (MB201-GRP)

The Msr family are well conserved proteins that function to reduce oxidized methionine to L-methionine. The SelR (MsrB) protein was identified as the enzyme responsible for reduction of the MICAL-oxidized Met 44 and Met47 amino acids of actin.. As part of the MOXtrue™ product line,human MSRB2 protein (MB201) [accession # Q9Y3D2 (MSRB2_HUMAN)] has been produced and purified from a bacterial expression system. The recombinant protein is N-terminally 6x Histidine tagged and is comprised of amino acids 42-182. Validation studies have shown that MB201 can reduce MICAL-oxidized actin which will enhance subtilisin A cleavage by >90% in limited proteolysis assays. MB201 has also been validated for the production of reduced actin which can be used in downstream applications such as sedimentation assays and actin polymerization assays.