MemGlow 400 Pro12A Blue Plasma-membrane Probe and Lipid Order/Disorder Probe
MemGlow 400 Pro12A Blue Plasma-membrane Probe and Lipid Order/Disorder Probe (MG09-GRP)
Products
Description
The MemGlow™ product line consists of bright & non-toxic live cell membrane probes. MemGlow™ fluorogenic probes exhibit ideal microscopy characteristics including high specificity, low background, and simple application1. MemGlow 400™ has been validated with multiple microscopy techniques including epifluorescent (widefield), confocal, 2-photon, and TIRF2. MemGlow™ has been confirmed to work in fixed cells, fixed tissue, live cells, and other phospholipid membranes such as extracellular vesicles including exosomes1-3.
Pro12A is an analogue of commonly used dye Laurdan, which was designed for specific labelling of cell plasma membrane outer leaflet. Owing to its solvatochromism, Pro12A can also be used to visualize lipid organization of cell membranes. Upon binding with a plasma membrane in a predominant liquid ordered (LO) phase Pro12A exhibits a 45-50 nm wavelength shift relative to liquid disordered (Ld) phase (Fig.3) enabling investigators to examine the nanoscale distribution of local chemical polarity in plasma membranes.
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** 16 nmoles of Pro12A will produce a 500 µM stock solution when reconstituted with 40 µl of anhydrous DMSO
Figure 4. A) Confocal imaging of live CHO cells labeled with 800 nM Pro12A, and imaged with a Zeiss LSM 780 confocal microscope using a 405 nm laser and 410-600 nm emission. B) Widefield epi-fluorescent imaging of live A431 cells labeled with 800 nM Pro12A, and imaged with a Dapi filter set.
Figure 1. Mechanism of action of MemGlow 400 Pro12A.
Figure 2. Excitation and emission spectra of MemGlow 400 Pro12A diluted to 5.0 µM. Absorbance peak detected at 363 nm, and peak emissions at 454 nm in DMSO, or 506 nm in water. Note lack of fluorescence above 650 nm.
Figure 3. Fluorescence spectra of Pro12A in DOPC (ordered), DOPC/cholesterol (disordered) and SM/cholesterol (disordered) phases (ref. 2).
Specifications
| Manufacturer | Cytoskeleton, Inc |
|---|---|
| Storage | 4°C |