H-Ras protein - His tagged - human wild type
H-Ras protein - His tagged - human wild type (RS01-GRP)
Products
Description
Product Uses Include
- Ras biochemistry
- Ras GTPase assays
- Ras nucleotide exchange assays
- Ras binding studies
Material
The human H-Ras protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.
The recombinant protein is approximately 28 kDa, consisting of the H-Ras protein plus a histidine tag in the amino-terminus.
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-H-Ras samples are >80% pure.
Biological Activity
The biological activity of His-H-Ras is determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to sequester magensium ions from the His-H-Ras protein, thereby stimulating nucleotide exchange activity. The RhoGEF exchange assay Biochem Kit™ (Cat. # BK100) is used to monitor the nucleotide exchange in His-H-Ras.
Specifications
| Storage | 4°C |
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