EnzyFluo™ NFkB Phosphorylation Assay KitEnzyFluo™ NFkB Phosphorylation Assay Kit
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EnzyFluo™ NFkB Phosphorylation Assay Kit

For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells.

• Simple and convenient. No cell lysis necessary, cells can be cultured for several days.

Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-α. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcription. BioAssay Systems’ cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.

Cat# Size Price Qty Buy
ENFKB-100 100 Tests £420.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells.
Method FL360/450nm, 530/585nm
Samples Cell, tissue etc
Species Human, mouse
Storage -20°C
Shelf Life 6 months

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