EnzyChrom™ Formate Assay KitEnzyChrom™ Formate Assay Kit
Move your mouse over image or click to enlarge

EnzyChrom™ Formate Assay Kit

Direct determination of formate concentrations in biological (e.g. urine, blood, etc.) and other samples. 

• Fast and sensitive. Use of 10 µL sample. Linear detection range 0.050 to 5 mM Formate in 96-well plate assay.

• Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed. 

• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

FORMATE (HCOO-) is the anion derived from formic acid, the simplest carboxylic acid. It is also the metabolic byproduct of formaldehyde metabolism in our body, and the eventual metabolic byproduct of methanol which is first broken down to formaldehyde. At high levels, formate is neurotoxic to the central nervous system and can cause blindness, coma, and death. Although naturally present in the body at low levels, elevated levels of formate may be used as an indicator of formaldehyde exposure and methanol poisoning.  BioAssay Systems' formate assay kit is based on formate dehydrogenase catalyzed oxidation of formate, which generates carbon dioxide and NADH that reduces a formazan (MTT) dye. The intensity of the reduced MTT, measured at 565 nm, is directly proportional to formate concentration in the sample.

Cat# Size Price Qty Buy
EFOR-100 100 Tests £386.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications Direct determination of formate concentrations in biological (e.g. urine, blood, etc.) and other samples. 
Method OD565nm
Samples Urine, Serum etc
Species All
Detection Limit 50 μM
Storage -20 °C
Shelf Life 6 months

Related Documents