EnzyChrom™ AF Cholesterol Assay KitEnzyChrom™ AF Cholesterol Assay Kit
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EnzyChrom™ AF Cholesterol Assay Kit

For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.

• Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays.

• Convenient. Room temperature assay. No 37°C heater is needed. 

• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

CHOLESTEROL is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. BioAssay Systems’ EnzyChrom™ Cholesterol Assay uses a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to total cholesterol concentration in the sample.

Cat# Size Price Qty Buy
E2CH-100 100 Tests £326.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.
Method OD570nm, or FL530/585nm
Samples Serum, plasma etc
Species All
Detection Limit OD, FL: 1, 0.2 mg/dL
Storage -20°C
Shelf Life 12 months
References Assay: Cholesterol in Mouse macrophage, membrane (Pubmed).

2. Lee, SM et al (2008).GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats. Lipids 43(5): 419-429. . Assay: Cholesterol in Rat blood (Pubmed).

3. Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210(1):57-62. Assay: Cholesterol in Mouse blood (Pubmed).

4. Guevara-Arauza, JC et al (2011). Biofunctional activity of tortillas and bars enhanced with nopal. Preliminary assessment of functional effect after intake on the oxidative status in healthy volunteers. Chem Cent J 5(1):10. Assay: Cholesterol in Human Plasma (Pubmed).

5. Uddin, MJ et al (2011). Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: Cholesterol in Pig Serum (Pubmed).

6. Stoll, C et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-81. Assay: Cholesterol in Human Red blood cells (Pubmed).

7. Waheed, MM et al (2011). Some Biochemical Characteristics and Preservation of Epididymal Camel Spermatozoa (Camelus dromedarius). Theriogenology . Assay: Cholesterol in Camel Epididymal fluid (Pubmed).

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1. Rub A et al (2009). Cholesterol depletion associated with Leishmania major infection alters macrophage CD40 signalosome composition and effector function. Nat Immunol. 10(3):273-80. Assay: Cholesterol in Mouse macrophage, membrane (Pubmed).

2. Lee, SM et al (2008).GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats. Lipids 43(5): 419-429. . Assay: Cholesterol in Rat blood (Pubmed).

3. Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210(1):57-62. Assay: Cholesterol in Mouse blood (Pubmed).

4. Guevara-Arauza, JC et al (2011). Biofunctional activity of tortillas and bars enhanced with nopal. Preliminary assessment of functional effect after intake on the oxidative status in healthy volunteers. Chem Cent J 5(1):10. Assay: Cholesterol in Human Plasma (Pubmed).

5. Uddin, MJ et al (2011). Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: Cholesterol in Pig Serum (Pubmed).

6. Stoll, C et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-81. Assay: Cholesterol in Human Red blood cells (Pubmed).

7. Waheed, MM et al (2011). Some Biochemical Characteristics and Preservation of Epididymal Camel Spermatozoa (Camelus dromedarius). Theriogenology . Assay: Cholesterol in Camel Epididymal fluid (Pubmed).

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1. Rub A et al (2009). Cholesterol depletion associated with Leishmania major infection alters macrophage CD40 signalosome composition and effector function. Nat Immunol. 10(3):273-80. Assay: Cholesterol in Mouse macrophage, membrane (Pubmed).

2. Lee, SM et al (2008).GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats. Lipids 43(5): 419-429. . Assay: Cholesterol in Rat blood (Pubmed).

3. Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210(1):57-62. Assay: Cholesterol in Mouse blood (Pubmed).

4. Guevara-Arauza, JC et al (2011). Biofunctional activity of tortillas and bars enhanced with nopal. Preliminary assessment of functional effect after intake on the oxidative status in healthy volunteers. Chem Cent J 5(1):10. Assay: Cholesterol in Human Plasma (Pubmed).

5. Uddin, MJ et al (2011). Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: Cholesterol in Pig Serum (Pubmed).

6. Stoll, C et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-81. Assay: Cholesterol in Human Red blood cells (Pubmed).

7. Waheed, MM et al (2011). Some Biochemical Characteristics and Preservation of Epididymal Camel Spermatozoa (Camelus dromedarius). Theriogenology . Assay: Cholesterol in Camel Epididymal fluid (Pubmed).

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