Endo S, recombinant lyophilized (36408.03)

Endo S, a recombinant endo-β-N-acetylglucosaminidase S from Streptococcus pyogenes, plays a central role in glycoengineering strategies for the development of IgG antibodies with improved therapeutic efficacy. This enzyme will leave any human IgG with a single N-acetylglucosamine, with or without an attached fucose molecule. Especially designed and tested for mass spectrometry imaging and HPLC/UPLC Outstanding performance — produces in HPLC clean peaks with very high yields Contains a His-tag for easy removal by affinity chromatography The endoglycosidase Endo S has a unique accuracy for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG molecules. The enzyme hydrolyzes the β(1-4) linkage between the two core GlcNAcs of asparagine linked biantennary complex-type glycans of human IgG Fc regions. Because the enzyme is lyophilized, there is no need for refrigerated transport and storage is at room temperature. Concentration after reconstitution: 200 u/µl (1.0 mg/ml) in 10 µl/50 µl/100 µl H2O dest. Delivered with 10x reaction buffer.