DryDrops PCR UDG, Single Assay PCR Lyophilized Beads (LP11514)

DryDrops®UDG lyophilized beads are a pre-prepared and packaged form of the polymerase chain reaction (PCR) reaction mixture. Uracil-DNA glycosylase excludes amplicon contamination from previous reactions and false-positive results. Each lyophilized PCR ball is a dry granule containing a ready-made reaction mixture with all the necessary components for PCR with a volume of 25 μL: hot-start Taq polymerase; uracil-DNA glycosylase (UDG); mixture of deoxynucleoside triphosphates (including dUTP); optimized PCR buffer (contains 3 mM Mg2+ in 1× of the reaction mixture); protectors for lyophilization. The composition of the reaction mixture is optimized to obtain ideal results in terms of processivity and amplification specificity. DryDrops® provide greater reproducibility between reactions by minimizing reaction preparation steps and reducing the potential for mixing errors and sample contamination. Each batch of DryDrops® undergoes functional testing to ensure reproducibility from batch to batch. DryDrops® are suitable for real-time PCR detection, including quantitative analysis (with fluorescent probes or intercalating dye, e.g., Eva488), as well as for DNA amplification followed by electrophoresis detection. For cloning tasks and other applications that require further work with the PCR product after amplification, we recommend using our dUTP-free DryDrops® PCR product. DryDrops® UDG lyophilized beads are compatible with all types of cyclers and can be used for PCR both in cyclers with classic thermoblocks for PCR tubes and in amplifiers with cartridges. We offer freeze-dried PCR beads in vials of 10 (11514) and vials of 50 (21514).