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Collagenase NB 4G Proved Grade

Collagenase NB 4G Proved Grade (S17465-GRP)

Nordmark Biochemicals
View Data Sheet
For Isolation of Hepatocytes, Fibroblasts and Epithelial Cells
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Description

Collagenase NB 4G Proved Grade is an enzyme mixture which contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The ratio of these activities leads to gentle and efficient tissue dissociation. The product is suitable for isolation of a broad variety of cell types, especially for fibroblasts, human and rodent hepatocytes, frog oocytes and epithelial cells.

Specifications

Manufacturer Nordmark Biochemicals
Storage +2 °C to +8 °C
References

References and Definitions

Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis
of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. 

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51