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Collagenase NB 4 Standard Grade

Nordmark Biochemicals

Collagenase NB 4 Standard Grade (S17454-GRP)

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For Dissociation of Cartilage, Umbilical Cord and More
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Description

Collagenase NB 4 Standard Grade is a natural enzyme mixture that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. As Collagenase NB 4 Standard Grade has comparable enzymatic properties to Collagenase NB 5 Sterile Grade and Collagenase NB 6 GMP Grade, this enzyme may also be used in order to establish applications which have to meet sterile or GMP requirements like tissue engineering and transplantation.

Specifications

Storage +2 °C to +8 °C
References

References and Definitions

Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51