Cdc42 G-LISA GTPase Activation Assay (Colorimetric format) (BK127-GRP)

Product Uses Include

• Cdc42 signaling pathway studies
• Cdc42 activation assays with primary cells
• Studies of Cdc42 activators and inactivators
• Cdc42 activation assays with limited material
• High throughput screens for Cdc42 activation

Introduction
The G-LISA® series of Small G-Protein Activation Assays are ELISA based assays with which you can measure the GTP form of small G-proteins from lysates of cells or tissues and all in less than 3 h. The Cdc42 G-LISA Activation Assay measures the entire level of GTP-loaded Cdc42 protein in cell lysates. The level of activation is measured by reading at OD490nm. For a kit to measure RhoA activation please check Cat.# BK124, for Rac1 go to Cat.# BK128 for Rac1,2,3 go to Cat.# BK125, for RalA go to Cat.# BK129.

The Cdc42 G-LISA Activation Assay is very sensitive and has excellent accuracy for duplicate samples.