Calcein AM, green fluorescent cell viability probe (LP3564)

Calcein AM is a cell-permeant green fluorogenic dye used to assess the viability of live cells. The neutral, hydrophobic Calcein AM easily crosses the cell membrane of living cells. Inside the cell, intracellular esterases cleave the acetoxymethyl (AM) group, releasing the Calcein molecule, which is a membrane-impermeant and highly fluorescent form. The intensity of the Calcein fluorescence is proportional to the number of living cells and the activity of the esterases within them. Calcein AM is used in microscopy and flow cytometry to indicate cell health. It is also a sensitive indicator for intracellular calcium changes, cytotoxicity, and multidrug resistance. Calcein AM staining is not compatible with cell fixation and permeabilization.