Biotin-11-ddUTP (LPS7040)

Biotin-11-ddUTP is a biotinylated dideoxyuridine triphosphate analog. Its dideoxy structure acts as an irreversible chain terminator during DNA synthesis, while the biotin moiety provides a stable tag for detection. The C11 spacer arm optimizes the molecule by facilitating polymerase recognition and ensuring unhindered interaction between the conjugated biotin and streptavidin. Biotin-11-ddUTP can be incorporated by many DNA polymerases and terminal transferases into growing DNA strands, where it irreversibly terminates elongation while introducing a single biotin tag at the incorporation site. This reagent is used for the enzymatic preparation of biotin-labeled oligonucleotide probes, end-labeling of DNA fragments, DNA sequencing, SNP detection, and probe labeling.