Arp2/3 protein complex - porcine brain (RP01P-GRP)

Product Uses Include

• Positive control for in vitro actin polymerization stimualting compounds
• Discovery of Arp2/3 activating or inhibiting compound
• Characterization and discovery of Arp2/3 interacting proteins

Material
The Arp2/3 protein complex consists of 7 protein subunits, all present in approximately equal stoichiometry (see Figure 1). The Arp2/3 complex is a key regulator of actin filament nucleation and the protein subunits are highly evolutionarily conserved.  The protein complex has been purified from porcine brain and is supplied as a lyophilized powder.  When reconstituted with distilled water, the complex is in the following buffer: 20 mM Tris pH 7.5, 25 mM KCl, 1 mM MgCl₂, 0.5 mM EDTA, 0.1 mM ATP, 1% dextran and 5% sucrose.  The molecular weight of the Arp2/3 complex is 224 kDa. 

Note: RP01P from procine brain replaces RP01 from bovine brain. We have compared the activity of Arp2/3 complex from both sources and have found them to behave identically in actin polymerization assays 

Purity
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gel. Arp2/3 protein complex is determined to be 90% pure.