Anti-RNase L - Purified <i>in vivo </i>GOLDâ„¢ Functional Grade
Anti-RNase L - Purified <i>in vivo </i>GOLDâ„¢ Functional Grade (R6000-GRP)
Products
Description
RNase L antibody, clone 2E9, recognizes the interferon (IFN)-inducible 2-5A-dependent ribonuclease L (RNase L), an endoribonuclease involved in antiviral signaling. It is widely expressed as a latent monomer in most mammalian tissues1. 2’,5’-oligoadenylate synthetase (OAS) is expressed following viral infection and exposure to IFN2. OAS proteins are activated by dsRNA, resulting in the production of 2’,5’-linked oligoadenylates (2-5A), which bind to latent RNase L, inducing dimerization and activation2. RNase L inhibits viral replication by cleaving viral and ribosomal RNA3,4 and inducing apoptosis in virus-infected cells5,6. RNase L is suggested to have antitumor effects, possibly through its pro-apoptotic activity7, and mutations in the RNASEL gene are associated with prostate cancer risk and progression8,9.
Specifications
| Manufacturer Cat# | R6000 |
|---|---|
| Concentration | ≥ 5.0 mg/ml |
| Formulation | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Alternate Names | RNase, RNA, ribonuclease L, 2',5'-oligoisoadenylate synthetase-dependent; 2-5A-dependent ribonuclease; 2-5A-dependent RNase; interferon-induced 2-5A-dependent RNase; Ribonuclease 4; Ribonuclease L; ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent); ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent); RNase L ; RNase L Antibody; 2e9; RNaseL Monoclonal Antibody; PRCA1; RNS4 |
| Isotype | IgG1 κ |
| Clone | 2000000000 |
| Target | RNaseL |
| Applications | ELISA, IHC FFPE, in vivo, WB |
| Antigen | RNase L is highly expressed in the spleen and thymus, followed by prostate, testis, uterus, small intestine, colon, and peripheral blood leukocytes. |
| Storage | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Protein | RNaseL |