Anti-Mouse TIM-4 - Purified in vivo PLATINUM™ Functional Grade (T832-GRP)

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity1. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma. RMT4-53 was generated by linking the extracellular domains of TIM-4 (aa 1-288) to the Fc portion of mouse IgG2a2. This protein product was then used to immunize Sprague Dawley rats. Subsequently, LN cells were fused with P3U1 myeloma cells. RMT4-53 reacts with TIM-4/NRK cells but not with parental NRK or other TIM family members. TIM-4 blockade has been investigated for the treatment of cancer3,4 and allograft rejection2,5 using anti-TIM-4 clone RMT4-53. Additionally, RMT4-53 blockade of TIM-4 leads to increased induction of iTregs from naïve CD4+ T cells2. Blockade with RMT4-53 has also been investigated in liver ischemia-reperfusion injury6,7.