Anti-Mouse TIM-2 - Biotin (T775-100ug)

The T cell/transmembrane, immunoglobulin, and mucin (TIM) family plays a critical role in immune response, transplant tolerance, autoimmunity, allergy and asthma regulation1. The TIM family consists of eight members, including TIM-2 which is only found in rodent genomes. TIM- 2 is likely a gene duplication of TIM-1. TIM-2 is a type I cell surface glycoprotein that has an N- terminal immunoglobulin-like (Ig) domain, a mucin domain with O- and N-linked glycosylations, a single transmembrane domain, and a cytoplasmic region with tyrosine phosphorylation motif. Unlike other TIM family proteins, TIM-2 does not bind PtdSer. TIM-2 is a receptor for H-ferritin. TIM-2 functions as a negative regulator of T cell activation and Th2 responses. RMT2-14 was generated by immunizing Sprague Dawley rats with a TIM-2-Ig chimera protein consisting of murine TIM-2-Ig (extracellular domain residues 1-230) and the Fc portion of mouse IgG2a2. LN cells were fused with P3U1 myeloma cells. The RMT2-14 hybridoma was selected using flow cytometry, based on reactivity to mouse TIM-2 transfected cells, but not parental cells. RMT2-14 inhibits binding of H-ferritin to TIM-2. RMT2-14 also significantly enhances B cell proliferation and activation in vitro by agonistic stimulation of TIM-2. RMT2-14 treatment exacerbates collagen-induced arthritis in DBA/1 mice, showing a pathophysiological role for TIM-2 in this autoimmune arthritis. RMT2-14 treatment did not affect the development or induction of Th1 and Th17 cells.