Anti-Mouse Thy1.1 (CD90.1) [Clone 19E12] - Purified <i>in vivo</i> GOLD™ Functional Grade (C3101-25mg)

Thy1 is a highly conserved, GPI-linked member of the immunoglobulin superfamily that is important in the immune and nervous systems1 and involved in T cell activation and cell-cell interactions2. The effects of Thy1 are context dependent1. Thy1 is heavily N-glycosylated with a carbohydrate content of up to 40% of its molecular mass, and its moiety composition varies between tissues as well as between cells of the same lineage in different stages of differentiation. Additionally, Thy1 is found in both membrane-bound and soluble forms, and, in mouse, Thy1 is encoded by two alleles, Thy1.1 and Thy1.2, which are distinguished by a single amino acid at position 891. Thy1 deficiency does not compromise immunity2, but its presence or absence modulates the phenotypes of certain cancers, fibrotic diseases, and neuronal injury1. Thy1.1 is an alloantigen of the AKR/J and PL mouse strains2. 19E12 was generated by immunizing 129 strain mice with allogeneic AKR SL3 leukemia cells and fusing the resulting lymphocytes with BALB/c MOPC21 NSI/1 myeloma cells3. 19E12 showed specificity for Thy1.1 antigen in cytotoxic assays. Additionally, 19E12 was found to have antitumor activity in (B6 x AKR)F1 hybrid mice, AKR parental mice3 and AKR/J mice4 inoculated with AKR SL2 cells. Antitumor activity was enhanced when administered in combination with rat monoclonal antibody R17 2085. 19E12 antibody has been used to create a bispecific hybrid antibody that can focus T cell activity against Thy1.1-expressing tumor cells for lysis in vitro6. A biotinylated form has also been used as a tumor pretargeting agent to increase the local concentration and persistence of human tumor necrosis factor alpha on a mouse tumor7. 19E12 can also be used to deplete Thy1.1-expressing cells in mice8,9,10,11,12, both in naturally occurring Thy1.1+ T cells and in cells trangenically expressing Thy1.1 due to experimental design. Thy1 is widely used as a marker for thymus T cells13, thymus-derived lymphocytes, and lipid rafts in murine T cells2.