Anti-mouse/rat MHC Class II (I-Ek/RT1-D) (Clone 14-4-4S (HB-32)) - FITC (H452-GRP)

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. Class II molecules, also known as Ia antigens, regulate recognition of foreign antigens on the surfaces of antigen presenting cells and play a major role in the mixed lymphocyte response2. Mice have two class II isotypes, I-A and I-E, each of which is a glycoprotein composed of an ⍺ and β Subunit. 14-4-4S was generated by immunizing C3H.SW mice with spleen cells from C3H mice3. The resulting spleen cells were then fused with Sp2/0-Ag14 myeloma cells for hybridoma generation. Further studies have found that whole 14-4-4S antibody and F(ab’)2 fragments block RT1-D-restricted Ag presentation as well as activation of an I-Eʹ-restricted T cell hybridoma in vitro4. Additionally, because 14-4-4S recognizes hamster B cells, 14-4-4S can be used in hamster to deplete B cells and enrich for T cells via flow cytometry5 or by removing adherent B lymphocytes in immunoglobulin-coated tissue culture dishes6.