Anti-Mouse MHC Class I (H-2Kk, H-2Dk) (Clone 15-3-1S) - Purified <i>in vivo</i> PLATINUM™ Functional Grade (H466-GRP)

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell. H-2Kk and H-2Dk are MHC class I proteins originally identified in the C3H mouse2. 15-3-1S was generated by immunizing C3H mice with spleen cells from C3H mice3. Subsequently, spleen cells from the immunized mice were fused with Sp2/0-Ag14 myeloma cells to create hybridoma lines. 15-3-1S was characterized by performing cytotoxic titer of hybridoma ascites on a panel of H-2 haplotypes4. Additionally, blocking ability and specificity were analyzed. 15-3-1S has relatively low affinity for H-2Kk according to radioactive binding assays with B10.A spleen cells.