Anti-Mouse MHC Class I (H-2Db) (Clone 28-14-8S) - Purified No Carrier Protein (H222-GRP)

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. H-2Db is a class I MHC cell surface protein. MHC class I molecules consist of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin)2,3. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins which are then displayed on the cell surface1 to CD8 T cells via a process known as cross-presentation4. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. For example, H-2Db plays a role in human papillomavirus infection5. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell1. 28-14-8S was generated by immunizing BALB/c C3H/HeJ (H-2k) mice with C3H.SW (H-2b) spleen cells6. Spleen cells were then fused with SP2/0 AG14 to create a hybridoma line. 28-14-8S does not react with Kd or Dd antigens. 28-14-8S binds to native molecules and free heavy chains7.