Anti-Mouse F4/80 [Clone Cl:A3-1] - Purified <i>in vivo </i>PLATINUM™ Functional Grade (F482-GRP)

Macrophages play an important role in innate and adaptive immune system functions1. F4/80 antigen is a widely used cell-surface marker of murine macrophages1, 2 encoded by the Adgre1 locus3. F4/80 antigen is a G protein-coupled receptor with an extracellular domain containing repeated Epidermal Growth Factor-like calcium-binding domains and as such is a member of the EGF-TM7 protein family, which itself is a subfamily of the adhesion G protein-coupled receptors (ADGRE) family. F4/80 is thought to have dual adhesion and signaling functions due to its receptor domains and is known to be required for the differentiation of antigen-specific CD8+ T regulatory cells1 . Adgre1 null mutant mice have a dysregulated autoimmune response and regulatory T cell generation3. Additionally, unstimulated resting macrophages express F4/80 at higher levels than activated macrophages1. Cl:A3-1 was generated by immunizing a rat with thioglycolate-induced peritoneal macrophages from C57BL/6 mice4. Spleen cells were fused with mouse myeloma cell line NS1. Cl:A3-1 does not bind to macrophages via Fc receptors and is not cytotoxic. Cl:A3-1 is able to modulate Listeria-induced cytokine signaling between macrophages and natural killer cells2.