Anti-Mouse CD90 (Thy-1) (Clone M5/49.4.1) - Purified <i>in vivo </i>PLATINUM™ Functional Grade (C645-GRP)

Thy1 is a highly conserved, GPI-linked member of the immunoglobulin superfamily that is important in the immune and nervous systems1 and involved in T-cell activation and cell-cell interactions2. The effects of Thy1 are context-dependent1. Thy1 is heavily N-glycosylated with a carbohydrate content of up to 40% of its molecular mass, and its moiety composition varies between tissues as well as between cells of the same lineage in different stages of differentiation. Additionally, Thy1 is found in both membrane-bound and soluble forms, and, in mice, Thy1 is encoded by two alleles, Thy1.1 and Thy1.2, which are distinguished by a single amino acid at position 891. Thy1 deficiency does not compromise immunity2, but its presence or absence modulates the phenotypes of certain cancers, fibrotic diseases, and neuronal injury1. Thy1.1 is an alloantigen of the AKR/J and PL mouse strains, whereas Thy1.2 is expressed by most mouse strains2,3. M5/49.4.1 was generated by immunizing (Lewis x BN) F1 rats with a secondary mixed lymphocyte culture consisting of C57BL/6 mouse anti-irradiated BN rat lymphoma4. Spleen cells were fused with NS-1 myeloma cells and hybridomas grown and assessed for binding to cytolytic T lymphocyte (CTL) surface molecules.