Anti-Mouse CD70 - APC (C2405-GRP)

CD70, a type II transmembrane glycoprotein of the TNF family with cytokine activity, is the ligand for the CD27 receptor1. Mouse CD70 (mCD70) has a potent co-stimulatory effect on T cell proliferation1 and acts as an immune-checkpoint2. In mouse, but not in humans, the CD70-CD27 axis mediates a negative feedback system that enables the immune system to modulate hematopoiesis2. Additionally, CD70-CD27 interactions are important to the formation of memory and plasma B cells. Immunotherapies that aim to eradicate tumor cells by targeting CD70 overexpression are being developed2. FR70 inhibits mCD70 binding to mCD27-Ig, a recombinant soluble form of mCD271, as well as to mCD27 in vitro3,4 and in vivo5. FR70 was produced by immunizing an F344/DuCrj rat with A20 cells and fusing the splenocytes with P3U1 cells to create hybridoma lines1. HAT selection was performed, and FR70 was identified by its strong reactivity against mCD70-BHK21 cells. Blocking with FR70 can prolong graft acceptance6,7. When mouse cardiac allogenic graft recipients are treated with FR70, tolerogenic dendritic cells and T regulatory cells are induced, resulting in decreased cytotoxic T lymphocyte proliferation as well as long-term graft acceptance6. Blocking with FR70 also prolongs mouse corneal graft survival7.